BD HorizonTM BV421 Mouse Anti-Human IL-8

Description

Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were primed for 2 hr with Recombinant Human IFN-gamma 10 ng/ml; Cat. No. 554617/554616 and stimulated with lipopolysaccharide 1.0 mug/ml; Sigma, Cat. No. L-8274; 6 hr in the presence of BD GolgiStop Protein Transport Inhibitor containing monensin Cat. No. 554724 . The PBMC were then stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Panel or BD Horizon BV421 Anti-Human IL-8 antibody Cat. No. 563310; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were primed for 2 hr with Recombinant Human IFN-gamma 10 ng/ml; Cat. No. 554617/554616 and stimulated with lipopolysaccharide 1.0 mug/ml; Sigma, Cat. No. L-8274; 6 hr in the presence of BD GolgiStop Protein Transport Inhibitor containing monensin Cat. No. 554724 . The PBMC were then stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Panel or BD Horizon BV421 Anti-Human IL-8 antibody Cat. No. 563310; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were primed for 2 hr with Recombinant Human IFN-gamma 10 ng/ml; Cat. No. 554617/554616 and stimulated with lipopolysaccharide 1.0 mug/ml; Sigma, Cat. No. L-8274; 6 hr in the presence of BD GolgiStop Protein Transport Inhibitor containing monensin Cat. No. 554724 . The PBMC were then stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Panel or BD Horizon BV421 Anti-Human IL-8 antibody Cat. No. 563310; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were primed for 2 hr with Recombinant Human IFN-gamma 10 ng/ml; Cat. No. 554617/554616 and stimulated with lipopolysaccharide 1.0 mug/ml; Sigma, Cat. No. L-8274; 6 hr in the presence of BD GolgiStop Protein Transport Inhibitor containing monensin Cat. No. 554724 . The PBMC were then stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Panel or BD Horizon BV421 Anti-Human IL-8 antibody Cat. No. 563310; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were primed for 2 hr with Recombinant Human IFN-gamma 10 ng/ml; Cat. No. 554617/554616 and stimulated with lipopolysaccharide 1.0 mug/ml; Sigma, Cat. No. L-8274; 6 hr in the presence of BD GolgiStop Protein Transport Inhibitor containing monensin Cat. No. 554724 . The PBMC were then stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Panel or BD Horizon BV421 Anti-Human IL-8 antibody Cat. No. 563310; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-8 expression in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were primed for 2 hr with Recombinant Human IFN-gamma 10 ng/ml; Cat. No. 554617/554616 and stimulated with lipopolysaccharide 1.0 mug/ml; Sigma, Cat. No. L-8274; 6 hr in the presence of BD GolgiStop Protein Transport Inhibitor containing monensin Cat. No. 554724 . The PBMC were then stained with APC Mouse Anti-Human CD14 antibody Cat. No. 555399/561708/561383 . After washing with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Panel or BD Horizon BV421 Anti-Human IL-8 antibody Cat. No. 563310; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of IL-8 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.