BD HorizonTM BV421 Mouse Anti-Human IL-9

Description

Flow cytometric analysis of IL-9 expressed in stimulated human lymphocytes. PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 10 ng/ml , IL-4 Cat. No. 554605; 50 ng/ml , and TGF-beta Corning; Cat. No. 354039; 10 ng/ml proteins and NA/LE Mouse Anti-Human IFN-gamma antibody Cat. No. 554698; 10 mug/ml . The cells were restimulated 5 h with PMA Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeablization Kit Cat. No. 554714 and stained with BD Horizon BV421 Mouse Anti-Human IL-9 antibody Cat. No. 564254 . A two-parameter flow cytometric contour plot showing the coexpressed levels of IL-9 versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRII System. Flow cytometric analysis of IL-9 expressed in stimulated human lymphocytes. PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 10 ng/ml , IL-4 Cat. No. 554605; 50 ng/ml , and TGF-beta Corning; Cat. No. 354039; 10 ng/ml proteins and NA/LE Mouse Anti-Human IFN-gamma antibody Cat. No. 554698; 10 mug/ml . The cells were restimulated 5 h with PMA Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeablization Kit Cat. No. 554714 and stained with BD Horizon BV421 Mouse Anti-Human IL-9 antibody Cat. No. 564254 . A two-parameter flow cytometric contour plot showing the coexpressed levels of IL-9 versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRII System. Show More Flow cytometric analysis of IL-9 expressed in stimulated human lymphocytes. PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 10 ng/ml , IL-4 Cat. No. 554605; 50 ng/ml , and TGF-beta Corning; Cat. No. 354039; 10 ng/ml proteins and NA/LE Mouse Anti-Human IFN-gamma antibody Cat. No. 554698; 10 mug/ml . The cells were restimulated 5 h with PMA Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeablization Kit Cat. No. 554714 and stained with BD Horizon BV421 Mouse Anti-Human IL-9 antibody Cat. No. 564254 . A two-parameter flow cytometric contour plot showing the coexpressed levels of IL-9 versus Autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRII System.