BD HorizonTM BV421 Mouse Anti-Human Ki-67

Description

Multiparameter flow cytometric analysis of Ki-67 expression in noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling normal Human peripheral blood mononuclear cells containing lymphocytes Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. In separate experiments, the normal or MOLT-4 cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plots or BD Horizon BV421 Mouse Anti-Human Ki-67 antibody Cat. No. 571657/571735; Right Plots and counterstained with BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DNA 7-AAD versus Ki-67 levels or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of Ki-67 expression in noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling normal Human peripheral blood mononuclear cells containing lymphocytes Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. In separate experiments, the normal or MOLT-4 cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plots or BD Horizon BV421 Mouse Anti-Human Ki-67 antibody Cat. No. 571657/571735; Right Plots and counterstained with BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DNA 7-AAD versus Ki-67 levels or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of Ki-67 expression in noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling normal Human peripheral blood mononuclear cells containing lymphocytes Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. In separate experiments, the normal or MOLT-4 cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plots or BD Horizon BV421 Mouse Anti-Human Ki-67 antibody Cat. No. 571657/571735; Right Plots and counterstained with BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DNA 7-AAD versus Ki-67 levels or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.