BD HorizonTM BV421 Mouse Anti-Human LOX-1 (OLR1)

Description

Flow cytometric analysis of LOX-1 OLR1 expression on viable GM-CSF-stimulated Human peripheral blood monocytes. Adherent Human peripheral blood mononuclear cells PBMC were cultured for 3 days with Recombinant Human GM-CSF protein Cat. No. 550068 . The cultured cells were harvested and stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram or BD Horizon BV421 Mouse Anti-Human LOX-1 OLR1 antibody Cat. No. 572005/572012; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing LOX-1 OLR1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of LOX-1 OLR1 expression on viable GM-CSF-stimulated Human peripheral blood monocytes. Adherent Human peripheral blood mononuclear cells PBMC were cultured for 3 days with Recombinant Human GM-CSF protein Cat. No. 550068 . The cultured cells were harvested and stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram or BD Horizon BV421 Mouse Anti-Human LOX-1 OLR1 antibody Cat. No. 572005/572012; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing LOX-1 OLR1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of LOX-1 OLR1 expression on viable GM-CSF-stimulated Human peripheral blood monocytes. Adherent Human peripheral blood mononuclear cells PBMC were cultured for 3 days with Recombinant Human GM-CSF protein Cat. No. 550068 . The cultured cells were harvested and stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram or BD Horizon BV421 Mouse Anti-Human LOX-1 OLR1 antibody Cat. No. 572005/572012; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing LOX-1 OLR1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.