BD HorizonTM BV421 Mouse Anti-Human Myeloperoxidase

Description

Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leukocyte populations. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The fixed leucocytes were washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human Myeloperoxidase antibody Cat. No. 570039/570120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Myeloperoxidase or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leukocyte populations. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The fixed leucocytes were washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human Myeloperoxidase antibody Cat. No. 570039/570120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Myeloperoxidase or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leukocyte populations. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The fixed leucocytes were washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human Myeloperoxidase antibody Cat. No. 570039/570120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Myeloperoxidase or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leukocyte populations. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The fixed leucocytes were washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human Myeloperoxidase antibody Cat. No. 570039/570120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Myeloperoxidase or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leukocyte populations. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The fixed leucocytes were washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human Myeloperoxidase antibody Cat. No. 570039/570120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Myeloperoxidase or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of Myeloperoxidase expression in Human peripheral blood leukocyte populations. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The fixed leucocytes were washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human Myeloperoxidase antibody Cat. No. 570039/570120; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Myeloperoxidase or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.