BD HorizonTM BV421 Mouse Anti-Human PD-L1 (CD274)

Description

Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568922/568923; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568922/568923; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568922/568923; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.