BD HorizonTM BV421 Mouse Anti-Human Pre-BCR

Description

Flow cytometric analysis of total Pre-BCR expression by 697 cells. Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with BD Horizon BV421 Mouse Anti-Human Pre-BCR antibody Cat. No. 570054/570135; solid line histogram at 0.25 mug/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human Pre-BCR antibody Cat. No. 570054/570135; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of total Pre-BCR expression by 697 cells. Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with BD Horizon BV421 Mouse Anti-Human Pre-BCR antibody Cat. No. 570054/570135; solid line histogram at 0.25 mug/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human Pre-BCR antibody Cat. No. 570054/570135; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of total Pre-BCR expression by 697 cells. Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with BD Horizon BV421 Mouse Anti-Human Pre-BCR antibody Cat. No. 570054/570135; solid line histogram at 0.25 mug/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human Pre-BCR antibody Cat. No. 570054/570135; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.