BD HorizonTM BV421 Mouse Anti-Human PSMA

Description

Flow cytometric analysis of PSMA expression on Human LNCaP cells. Cells from the Human LNCaP Prostate Carcinoma, ATCC CRL-1740 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human PSMA antibody Cat. No. 570315/570393; solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing PSMA expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD -negative LNCaP cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of PSMA expression on Human LNCaP cells. Cells from the Human LNCaP Prostate Carcinoma, ATCC CRL-1740 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human PSMA antibody Cat. No. 570315/570393; solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing PSMA expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD -negative LNCaP cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of PSMA expression on Human LNCaP cells. Cells from the Human LNCaP Prostate Carcinoma, ATCC CRL-1740 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human PSMA antibody Cat. No. 570315/570393; solid line histogram at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing PSMA expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD -negative LNCaP cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.