BD HorizonTM BV421 Mouse Anti-Human SIT

Description

Two-color flow cytometric analysis of SIT expression in Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BB515 Mouse Anti-Human CD19 Cat. No. 564456 . After staining, cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 SIT antibody Cat. No. 569799/569873 . The bivariate pseudocolor density plot showing the correlated expression of SIT or Ig Isotype control staining versus CD19 signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo Software. Two-color flow cytometric analysis of SIT expression in Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BB515 Mouse Anti-Human CD19 Cat. No. 564456 . After staining, cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 SIT antibody Cat. No. 569799/569873 . The bivariate pseudocolor density plot showing the correlated expression of SIT or Ig Isotype control staining versus CD19 signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo Software. Two-color flow cytometric analysis of SIT expression in Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BB515 Mouse Anti-Human CD19 Cat. No. 564456 . After staining, cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 SIT antibody Cat. No. 569799/569873 . The bivariate pseudocolor density plot showing the correlated expression of SIT or Ig Isotype control staining versus CD19 signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo Software. Show More Two-color flow cytometric analysis of SIT expression in Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BB515 Mouse Anti-Human CD19 Cat. No. 564456 . After staining, cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 SIT antibody Cat. No. 569799/569873 . The bivariate pseudocolor density plot showing the correlated expression of SIT or Ig Isotype control staining versus CD19 signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo Software. Show More Two-color flow cytometric analysis of SIT expression in Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BB515 Mouse Anti-Human CD19 Cat. No. 564456 . After staining, cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 SIT antibody Cat. No. 569799/569873 . The bivariate pseudocolor density plot showing the correlated expression of SIT or Ig Isotype control staining versus CD19 signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo Software. Two-color flow cytometric analysis of SIT expression in Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BB515 Mouse Anti-Human CD19 Cat. No. 564456 . After staining, cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized using BD Perm/Wash Buffer Cat. No. 554723 and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 SIT antibody Cat. No. 569799/569873 . The bivariate pseudocolor density plot showing the correlated expression of SIT or Ig Isotype control staining versus CD19 signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo Software.