
569639 ·
BDB Bioscience · Cat: 569639
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Description
Two-color flow cytometric analysis of TCR Vbeta23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta23 antibody Cat. No. 569639; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta23 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of TCR Vbeta23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta23 antibody Cat. No. 569639; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta23 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of TCR Vbeta23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta23 antibody Cat. No. 569639; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta23 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of TCR Vbeta23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta23 antibody Cat. No. 569639; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta23 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of TCR Vbeta23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta23 antibody Cat. No. 569639; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta23 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of TCR Vbeta23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta23 antibody Cat. No. 569639; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta23 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.