BD HorizonTM BV421 Mouse Anti-Human TCR Vbeta5.2/5.3

Description

Two-color flow cytometric analysis of TCR Vbeta5.2/5.3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5.2/5.3 antibody Cat. No. 567971/568023; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.2/5.3 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of TCR Vbeta5.2/5.3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5.2/5.3 antibody Cat. No. 567971/568023; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.2/5.3 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of TCR Vbeta5.2/5.3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5.2/5.3 antibody Cat. No. 567971/568023; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.2/5.3 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Two-color flow cytometric analysis of TCR Vbeta5.2/5.3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5.2/5.3 antibody Cat. No. 567971/568023; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.2/5.3 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Two-color flow cytometric analysis of TCR Vbeta5.2/5.3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5.2/5.3 antibody Cat. No. 567971/568023; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.2/5.3 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of TCR Vbeta5.2/5.3 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5.2/5.3 antibody Cat. No. 567971/568023; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . A bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5.2/5.3 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software.