BD HorizonTM BV421 Mouse Anti-Human TCR Vbeta5 Related

Description

Two-color flow cytometric analysis of TCR Vbeta5 Related expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD3 antibody Cat. No. 562280; Top Plots and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5 Related antibody Cat. No. 570064/570145; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5 Related or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of TCR Vbeta5 Related expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD3 antibody Cat. No. 562280; Top Plots and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5 Related antibody Cat. No. 570064/570145; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5 Related or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of TCR Vbeta5 Related expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD3 antibody Cat. No. 562280; Top Plots and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plot or BD Horizon BV421 Mouse Anti-Human TCR Vbeta5 Related antibody Cat. No. 570064/570145; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta5 Related or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.