BD HorizonTM BV421 Mouse Anti-Human uPA (PLAU)

Description

Flow cytometric analysis of uPA PLAU expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos Burkitt Lymphoma, ATCC CRL-1596 Left Plot or Human HT-1080 Fibrosarcoma, ATCC CCL-121 Right Plot cell lines were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human uPA PLAU antibody Cat. No. 570389/570459; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing uPA PLAU expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of uPA PLAU expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos Burkitt Lymphoma, ATCC CRL-1596 Left Plot or Human HT-1080 Fibrosarcoma, ATCC CCL-121 Right Plot cell lines were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human uPA PLAU antibody Cat. No. 570389/570459; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing uPA PLAU expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of uPA PLAU expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos Burkitt Lymphoma, ATCC CRL-1596 Left Plot or Human HT-1080 Fibrosarcoma, ATCC CCL-121 Right Plot cell lines were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human uPA PLAU antibody Cat. No. 570389/570459; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing uPA PLAU expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of uPA PLAU expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos Burkitt Lymphoma, ATCC CRL-1596 Left Plot or Human HT-1080 Fibrosarcoma, ATCC CCL-121 Right Plot cell lines were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human uPA PLAU antibody Cat. No. 570389/570459; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing uPA PLAU expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of uPA PLAU expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos Burkitt Lymphoma, ATCC CRL-1596 Left Plot or Human HT-1080 Fibrosarcoma, ATCC CCL-121 Right Plot cell lines were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human uPA PLAU antibody Cat. No. 570389/570459; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing uPA PLAU expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of uPA PLAU expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos Burkitt Lymphoma, ATCC CRL-1596 Left Plot or Human HT-1080 Fibrosarcoma, ATCC CCL-121 Right Plot cell lines were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human uPA PLAU antibody Cat. No. 570389/570459; solid line histograms . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing uPA PLAU expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.