BD HorizonTM BV421 Mouse Anti-Ki-67

Description

Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling Human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with BD Horizon BV421 Mouse Anti-Ki-67 antibody Cat. No. 562899/565929 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling Human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with BD Horizon BV421 Mouse Anti-Ki-67 antibody Cat. No. 562899/565929 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling Human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with BD Horizon BV421 Mouse Anti-Ki-67 antibody Cat. No. 562899/565929 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling Human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with BD Horizon BV421 Mouse Anti-Ki-67 antibody Cat. No. 562899/565929 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling Human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with BD Horizon BV421 Mouse Anti-Ki-67 antibody Cat. No. 562899/565929 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling Human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with BD Horizon BV421 Mouse Anti-Ki-67 antibody Cat. No. 562899/565929 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System.