BD HorizonTM BV421 Mouse Anti-Mouse CD28

Description

Two-color flow cytometric analysis of CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Plot or BD Horizon BV421 Mouse Anti-CD28 antibody Cat. No. 566816; Right Plot at 0.5 mug/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Plot or BD Horizon BV421 Mouse Anti-CD28 antibody Cat. No. 566816; Right Plot at 0.5 mug/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Plot or BD Horizon BV421 Mouse Anti-CD28 antibody Cat. No. 566816; Right Plot at 0.5 mug/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Plot or BD Horizon BV421 Mouse Anti-CD28 antibody Cat. No. 566816; Right Plot at 0.5 mug/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Plot or BD Horizon BV421 Mouse Anti-CD28 antibody Cat. No. 566816; Right Plot at 0.5 mug/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; Left Plot or BD Horizon BV421 Mouse Anti-CD28 antibody Cat. No. 566816; Right Plot at 0.5 mug/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.