BD HorizonTM BV421 Mouse Anti-Mouse FoxP3

Description

Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. C57BL/6 mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553729 , BD Horizon BUV395 Rat Anti-Mouse CD25 antibody Cat. No. 564022 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Top Plots or BD Horizon BV421 Mouse Anti-Foxp3 antibody Cat. No. 567458; Bottom plots at 0.5 mug/test. Left Plots: Bivariate pseudocolor density plots showing the correlated expression of CD4 versus Foxp3 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Right Plots: Bivariate pseudocolor density plots showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD25 were derived from CD4 positive-gated events with the light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. C57BL/6 mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553729 , BD Horizon BUV395 Rat Anti-Mouse CD25 antibody Cat. No. 564022 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Top Plots or BD Horizon BV421 Mouse Anti-Foxp3 antibody Cat. No. 567458; Bottom plots at 0.5 mug/test. Left Plots: Bivariate pseudocolor density plots showing the correlated expression of CD4 versus Foxp3 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Right Plots: Bivariate pseudocolor density plots showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD25 were derived from CD4 positive-gated events with the light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. C57BL/6 mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553729 , BD Horizon BUV395 Rat Anti-Mouse CD25 antibody Cat. No. 564022 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Top Plots or BD Horizon BV421 Mouse Anti-Foxp3 antibody Cat. No. 567458; Bottom plots at 0.5 mug/test. Left Plots: Bivariate pseudocolor density plots showing the correlated expression of CD4 versus Foxp3 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Right Plots: Bivariate pseudocolor density plots showing the correlated expression of Foxp3 or Ig Isotype control staining versus CD25 were derived from CD4 positive-gated events with the light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.