BD HorizonTM BV421 Mouse Anti-Mouse H-2K[b]/SIINFEKL

Description

Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570013/570094; solid line histograms at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with prior SIINFEKL peptide treatment. Mouse splenic leukocytes were pulsed with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570013/570094; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of H-2K b /SIINFEKL or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative splenic leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570013/570094; solid line histograms at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with prior SIINFEKL peptide treatment. Mouse splenic leukocytes were pulsed with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570013/570094; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of H-2K b /SIINFEKL or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative splenic leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570013/570094; solid line histograms at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with prior SIINFEKL peptide treatment. Mouse splenic leukocytes were pulsed with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570013/570094; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of H-2K b /SIINFEKL or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative splenic leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.