BD HorizonTM BV421 Mouse Anti-Mouse LAP

Description

Two-color flow cytometric analysis of LAP expression on activated mouse CD4+ T cells. BALB/c mouse splenic leucocytes were activated in culture 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057 and Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294 antibodies in the presence of Natural Transforming Growth Factor-b protein TGF-b; Corning Cat. No. CB-40039 . The cells were harvested and stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730/557308/561829 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse LAP antibody Cat. No. 565638; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of LAP expression on activated mouse CD4+ T cells. BALB/c mouse splenic leucocytes were activated in culture 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057 and Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294 antibodies in the presence of Natural Transforming Growth Factor-b protein TGF-b; Corning Cat. No. CB-40039 . The cells were harvested and stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730/557308/561829 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse LAP antibody Cat. No. 565638; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of LAP expression on activated mouse CD4+ T cells. BALB/c mouse splenic leucocytes were activated in culture 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057 and Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294 antibodies in the presence of Natural Transforming Growth Factor-b protein TGF-b; Corning Cat. No. CB-40039 . The cells were harvested and stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730/557308/561829 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse LAP antibody Cat. No. 565638; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of LAP expression on activated mouse CD4+ T cells. BALB/c mouse splenic leucocytes were activated in culture 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057 and Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294 antibodies in the presence of Natural Transforming Growth Factor-b protein TGF-b; Corning Cat. No. CB-40039 . The cells were harvested and stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730/557308/561829 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse LAP antibody Cat. No. 565638; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of LAP expression on activated mouse CD4+ T cells. BALB/c mouse splenic leucocytes were activated in culture 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057 and Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294 antibodies in the presence of Natural Transforming Growth Factor-b protein TGF-b; Corning Cat. No. CB-40039 . The cells were harvested and stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730/557308/561829 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse LAP antibody Cat. No. 565638; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of LAP expression on activated mouse CD4+ T cells. BALB/c mouse splenic leucocytes were activated in culture 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057 and Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294 antibodies in the presence of Natural Transforming Growth Factor-b protein TGF-b; Corning Cat. No. CB-40039 . The cells were harvested and stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553730/557308/561829 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse LAP antibody Cat. No. 565638; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System.