BD HorizonTM BV421 Mouse Anti-Mouse TCR Vgamma4

Description

Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic gammadelta T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse gammadelta T-Cell Receptor antibody Cat. No. 553178 , APC Hamster Anti-Mouse CD3e Cat. 553066/561826 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569444/569445; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative CD3-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic gammadelta T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse gammadelta T-Cell Receptor antibody Cat. No. 553178 , APC Hamster Anti-Mouse CD3e Cat. 553066/561826 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569444/569445; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative CD3-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic gammadelta T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse gammadelta T-Cell Receptor antibody Cat. No. 553178 , APC Hamster Anti-Mouse CD3e Cat. 553066/561826 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569444/569445; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative CD3-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic gammadelta T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse gammadelta T-Cell Receptor antibody Cat. No. 553178 , APC Hamster Anti-Mouse CD3e Cat. 553066/561826 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569444/569445; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative CD3-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic gammadelta T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse gammadelta T-Cell Receptor antibody Cat. No. 553178 , APC Hamster Anti-Mouse CD3e Cat. 553066/561826 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569444/569445; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative CD3-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic gammadelta T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse gammadelta T-Cell Receptor antibody Cat. No. 553178 , APC Hamster Anti-Mouse CD3e Cat. 553066/561826 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569444/569445; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative CD3-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.