BD HorizonTM BV421 Mouse Anti-Oct3/4

Description

Flow cytometric analysis and immunofluorescent staining of Oct3/4 expression in human embryonic stem ES cells. LEFT: H9 human ES cells WiCell, Madison, WI grown in mTESR1 media StemCell Technologies were harvested using Accutase Cell Detachment Solution Cat. No. 561527 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with either BD Horizon BV421 Mouse anti-Oct3/4 Cat. No. 565644, solid line or BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. RIGHT: H9 human ES cells WiCell, Madison, WI were cultured in a 96-well imaging plate, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Human Oct3/4 antibody Cat. No. 565644, pseudo-colored red and counterstained with BD Pharmingen DRAQ5 Cat. No. 564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20. Flow cytometric analysis and immunofluorescent staining of Oct3/4 expression in human embryonic stem ES cells. LEFT: H9 human ES cells WiCell, Madison, WI grown in mTESR1 media StemCell Technologies were harvested using Accutase Cell Detachment Solution Cat. No. 561527 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with either BD Horizon BV421 Mouse anti-Oct3/4 Cat. No. 565644, solid line or BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. RIGHT: H9 human ES cells WiCell, Madison, WI were cultured in a 96-well imaging plate, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Human Oct3/4 antibody Cat. No. 565644, pseudo-colored red and counterstained with BD Pharmingen DRAQ5 Cat. No. 564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20. Flow cytometric analysis and immunofluorescent staining of Oct3/4 expression in human embryonic stem ES cells. LEFT: H9 human ES cells WiCell, Madison, WI grown in mTESR1 media StemCell Technologies were harvested using Accutase Cell Detachment Solution Cat. No. 561527 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with either BD Horizon BV421 Mouse anti-Oct3/4 Cat. No. 565644, solid line or BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. RIGHT: H9 human ES cells WiCell, Madison, WI were cultured in a 96-well imaging plate, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Human Oct3/4 antibody Cat. No. 565644, pseudo-colored red and counterstained with BD Pharmingen DRAQ5 Cat. No. 564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20. Show More Flow cytometric analysis and immunofluorescent staining of Oct3/4 expression in human embryonic stem ES cells. LEFT: H9 human ES cells WiCell, Madison, WI grown in mTESR1 media StemCell Technologies were harvested using Accutase Cell Detachment Solution Cat. No. 561527 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with either BD Horizon BV421 Mouse anti-Oct3/4 Cat. No. 565644, solid line or BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. RIGHT: H9 human ES cells WiCell, Madison, WI were cultured in a 96-well imaging plate, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Human Oct3/4 antibody Cat. No. 565644, pseudo-colored red and counterstained with BD Pharmingen DRAQ5 Cat. No. 564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20. Show More Flow cytometric analysis and immunofluorescent staining of Oct3/4 expression in human embryonic stem ES cells. LEFT: H9 human ES cells WiCell, Madison, WI grown in mTESR1 media StemCell Technologies were harvested using Accutase Cell Detachment Solution Cat. No. 561527 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with either BD Horizon BV421 Mouse anti-Oct3/4 Cat. No. 565644, solid line or BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. RIGHT: H9 human ES cells WiCell, Madison, WI were cultured in a 96-well imaging plate, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Human Oct3/4 antibody Cat. No. 565644, pseudo-colored red and counterstained with BD Pharmingen DRAQ5 Cat. No. 564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20. Flow cytometric analysis and immunofluorescent staining of Oct3/4 expression in human embryonic stem ES cells. LEFT: H9 human ES cells WiCell, Madison, WI grown in mTESR1 media StemCell Technologies were harvested using Accutase Cell Detachment Solution Cat. No. 561527 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . Cells were stained with either BD Horizon BV421 Mouse anti-Oct3/4 Cat. No. 565644, solid line or BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. RIGHT: H9 human ES cells WiCell, Madison, WI were cultured in a 96-well imaging plate, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Human Oct3/4 antibody Cat. No. 565644, pseudo-colored red and counterstained with BD Pharmingen DRAQ5 Cat. No. 564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20.