BD HorizonTM BV421 Mouse Anti-Rat CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated rat splenocytes. Left and Middle Plots: Freshly-prepared, unstimulated rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with PE Mouse Anti-Rat CD4 antibody Cat. No. 554838 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Rat CD25 antibody Cat. No. 565608; Middle Plot . Two-color flow cytometric dot plots showing the correlated expression pattern of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Plot: Rat splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV421 Mouse Anti-Rat CD25 antibody solid line histogram . The normalized fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated rat splenocytes. Left and Middle Plots: Freshly-prepared, unstimulated rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with PE Mouse Anti-Rat CD4 antibody Cat. No. 554838 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Rat CD25 antibody Cat. No. 565608; Middle Plot . Two-color flow cytometric dot plots showing the correlated expression pattern of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Plot: Rat splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV421 Mouse Anti-Rat CD25 antibody solid line histogram . The normalized fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated rat splenocytes. Left and Middle Plots: Freshly-prepared, unstimulated rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with PE Mouse Anti-Rat CD4 antibody Cat. No. 554838 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Rat CD25 antibody Cat. No. 565608; Middle Plot . Two-color flow cytometric dot plots showing the correlated expression pattern of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Plot: Rat splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV421 Mouse Anti-Rat CD25 antibody solid line histogram . The normalized fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated rat splenocytes. Left and Middle Plots: Freshly-prepared, unstimulated rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with PE Mouse Anti-Rat CD4 antibody Cat. No. 554838 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Rat CD25 antibody Cat. No. 565608; Middle Plot . Two-color flow cytometric dot plots showing the correlated expression pattern of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Plot: Rat splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV421 Mouse Anti-Rat CD25 antibody solid line histogram . The normalized fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated rat splenocytes. Left and Middle Plots: Freshly-prepared, unstimulated rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with PE Mouse Anti-Rat CD4 antibody Cat. No. 554838 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Rat CD25 antibody Cat. No. 565608; Middle Plot . Two-color flow cytometric dot plots showing the correlated expression pattern of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Plot: Rat splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV421 Mouse Anti-Rat CD25 antibody solid line histogram . The normalized fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated rat splenocytes. Left and Middle Plots: Freshly-prepared, unstimulated rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with PE Mouse Anti-Rat CD4 antibody Cat. No. 554838 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Rat CD25 antibody Cat. No. 565608; Middle Plot . Two-color flow cytometric dot plots showing the correlated expression pattern of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Plot: Rat splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV421 Mouse Anti-Rat CD25 antibody solid line histogram . The normalized fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.