BD HorizonTM BV421 Mouse Anti-RUNX3

Description

Multicolor flow cytometric analysis of RUNX3 expression in human peripheral blood cells and mouse lymph node cells. Panel 1. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD8alpha Cat. No. 563796 , FITC Mouse Anti-Human CD3 Cat. No. 555332/561806/561807 and APC Mouse Anti-Human CD56 Cat. No. 555518 antibodies. The cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD HorizonBV421 Mouse Anti-RUNX3 antibody Cat. No. 565742 . The bivariate pseudocolor density plots on the left show the correlated expression of Ig Isotype control staining Upper Left Plot or RUNX3 Bottom Left Plot versus CD8alpha for gated events with the light scattering characteristics of intact lymphocytes. Intact cells were also gated as indicated Upper Right Plot and used for the generation of the overlaid dot plot figure Bottom Right Plot showing the correlated expression of RUNX3 versus CD3 for the CD8+CD56+ blue , CD8+CD56- green , and CD8-CD56+ red cell subsets. Panel 2. C57BL/6 mouse lymph node cells LNC were stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 and then fixed and permeabilized as described above , followed by staining with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Upper Plot or BD Horizon BV421 Mouse Anti-RUNX3 antibody Bottom Plot . The bivariate pseudocolor density plots showing the correlated expression of RUNX3 or Ig Isotype control staining versus CD4 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Multicolor flow cytometric analysis of RUNX3 expression in human peripheral blood cells and mouse lymph node cells. Panel 1. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD8alpha Cat. No. 563796 , FITC Mouse Anti-Human CD3 Cat. No. 555332/561806/561807 and APC Mouse Anti-Human CD56 Cat. No. 555518 antibodies. The cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD HorizonBV421 Mouse Anti-RUNX3 antibody Cat. No. 565742 . The bivariate pseudocolor density plots on the left show the correlated expression of Ig Isotype control staining Upper Left Plot or RUNX3 Bottom Left Plot versus CD8alpha for gated events with the light scattering characteristics of intact lymphocytes. Intact cells were also gated as indicated Upper Right Plot and used for the generation of the overlaid dot plot figure Bottom Right Plot showing the correlated expression of RUNX3 versus CD3 for the CD8+CD56+ blue , CD8+CD56- green , and CD8-CD56+ red cell subsets. Panel 2. C57BL/6 mouse lymph node cells LNC were stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 and then fixed and permeabilized as described above , followed by staining with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Upper Plot or BD Horizon BV421 Mouse Anti-RUNX3 antibody Bottom Plot . The bivariate pseudocolor density plots showing the correlated expression of RUNX3 or Ig Isotype control staining versus CD4 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Show More Multicolor flow cytometric analysis of RUNX3 expression in human peripheral blood cells and mouse lymph node cells. Panel 1. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD8alpha Cat. No. 563796 , FITC Mouse Anti-Human CD3 Cat. No. 555332/561806/561807 and APC Mouse Anti-Human CD56 Cat. No. 555518 antibodies. The cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD HorizonBV421 Mouse Anti-RUNX3 antibody Cat. No. 565742 . The bivariate pseudocolor density plots on the left show the correlated expression of Ig Isotype control staining Upper Left Plot or RUNX3 Bottom Left Plot versus CD8alpha for gated events with the light scattering characteristics of intact lymphocytes. Intact cells were also gated as indicated Upper Right Plot and used for the generation of the overlaid dot plot figure Bottom Right Plot showing the correlated expression of RUNX3 versus CD3 for the CD8+CD56+ blue , CD8+CD56- green , and CD8-CD56+ red cell subsets. Panel 2. C57BL/6 mouse lymph node cells LNC were stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553048/553049/561837 and then fixed and permeabilized as described above , followed by staining with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Upper Plot or BD Horizon BV421 Mouse Anti-RUNX3 antibody Bottom Plot . The bivariate pseudocolor density plots showing the correlated expression of RUNX3 or Ig Isotype control staining versus CD4 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System.