BD HorizonTM BV421 Mouse Anti-TCF-7/TCF-1

Description

Two-color flow cytometric analysis of TCF-7/TCF-1 expression by Human lymphocytes or Mouse splenocytes. Upper Plots - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 566692; Right Plot at 0.125 mug/test. Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BV421 Mouse Anti-TCF-7/TCF-1 antibody Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF-7/TCF-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCF-7/TCF-1 expression by Human lymphocytes or Mouse splenocytes. Upper Plots - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 566692; Right Plot at 0.125 mug/test. Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BV421 Mouse Anti-TCF-7/TCF-1 antibody Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF-7/TCF-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCF-7/TCF-1 expression by Human lymphocytes or Mouse splenocytes. Upper Plots - Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-TCF-7/TCF-1 antibody Cat. No. 566692; Right Plot at 0.125 mug/test. Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051/561091 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BV421 Mouse Anti-TCF-7/TCF-1 antibody Right Plot at 0.125 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF-7/TCF-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.