BD HorizonTM BV421 Mouse Anti-XBP-1S

Description

Two-color flow cytometric analysis of XBP-1S expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide LPS; Sigma L-3137; 1 mug/ml for 3 days 37C . Cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-XBP-1S antibody Cat. No. 563382 . Two-color flow cytometric dot plots show the correlated expression of CD45R/B220 versus XBP-1S or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of XBP-1S expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide LPS; Sigma L-3137; 1 mug/ml for 3 days 37C . Cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-XBP-1S antibody Cat. No. 563382 . Two-color flow cytometric dot plots show the correlated expression of CD45R/B220 versus XBP-1S or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of XBP-1S expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide LPS; Sigma L-3137; 1 mug/ml for 3 days 37C . Cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-XBP-1S antibody Cat. No. 563382 . Two-color flow cytometric dot plots show the correlated expression of CD45R/B220 versus XBP-1S or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of XBP-1S expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide LPS; Sigma L-3137; 1 mug/ml for 3 days 37C . Cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-XBP-1S antibody Cat. No. 563382 . Two-color flow cytometric dot plots show the correlated expression of CD45R/B220 versus XBP-1S or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of XBP-1S expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide LPS; Sigma L-3137; 1 mug/ml for 3 days 37C . Cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-XBP-1S antibody Cat. No. 563382 . Two-color flow cytometric dot plots show the correlated expression of CD45R/B220 versus XBP-1S or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of XBP-1S expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated with lipopolysaccharide LPS; Sigma L-3137; 1 mug/ml for 3 days 37C . Cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-XBP-1S antibody Cat. No. 563382 . Two-color flow cytometric dot plots show the correlated expression of CD45R/B220 versus XBP-1S or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.