BD HorizonTM BV421 Rat Anti-Human Clec9A (CD370)

Description

Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Human Clec9A CD370 antibody Cat. No. 564266; Right Panel . The cells were also stained with PE Mouse Anti-Human HLA-DR Cat No. 556644/555812/560943 and BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 antibodies and FITC Lineage Cocktail 1 LIN 1 - CD3, CD14, CD16, CD19, CD20, CD56 Cat. No. 340546 - a lineage cocktail comprised of FITC-conjugated antibodies for the specified CD markers. Two-color flow cytometric dot plots showing the correlated expression CD141 versus Clec9A CD370 or Ig Isotype control staining were derived from Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Human Clec9A CD370 antibody Cat. No. 564266; Right Panel . The cells were also stained with PE Mouse Anti-Human HLA-DR Cat No. 556644/555812/560943 and BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 antibodies and FITC Lineage Cocktail 1 LIN 1 - CD3, CD14, CD16, CD19, CD20, CD56 Cat. No. 340546 - a lineage cocktail comprised of FITC-conjugated antibodies for the specified CD markers. Two-color flow cytometric dot plots showing the correlated expression CD141 versus Clec9A CD370 or Ig Isotype control staining were derived from Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Human Clec9A CD370 antibody Cat. No. 564266; Right Panel . The cells were also stained with PE Mouse Anti-Human HLA-DR Cat No. 556644/555812/560943 and BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 antibodies and FITC Lineage Cocktail 1 LIN 1 - CD3, CD14, CD16, CD19, CD20, CD56 Cat. No. 340546 - a lineage cocktail comprised of FITC-conjugated antibodies for the specified CD markers. Two-color flow cytometric dot plots showing the correlated expression CD141 versus Clec9A CD370 or Ig Isotype control staining were derived from Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Human Clec9A CD370 antibody Cat. No. 564266; Right Panel . The cells were also stained with PE Mouse Anti-Human HLA-DR Cat No. 556644/555812/560943 and BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 antibodies and FITC Lineage Cocktail 1 LIN 1 - CD3, CD14, CD16, CD19, CD20, CD56 Cat. No. 340546 - a lineage cocktail comprised of FITC-conjugated antibodies for the specified CD markers. Two-color flow cytometric dot plots showing the correlated expression CD141 versus Clec9A CD370 or Ig Isotype control staining were derived from Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Human Clec9A CD370 antibody Cat. No. 564266; Right Panel . The cells were also stained with PE Mouse Anti-Human HLA-DR Cat No. 556644/555812/560943 and BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 antibodies and FITC Lineage Cocktail 1 LIN 1 - CD3, CD14, CD16, CD19, CD20, CD56 Cat. No. 340546 - a lineage cocktail comprised of FITC-conjugated antibodies for the specified CD markers. Two-color flow cytometric dot plots showing the correlated expression CD141 versus Clec9A CD370 or Ig Isotype control staining were derived from Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Human Clec9A CD370 antibody Cat. No. 564266; Right Panel . The cells were also stained with PE Mouse Anti-Human HLA-DR Cat No. 556644/555812/560943 and BD Horizon BV711 Mouse Anti-Human CD141 Cat. No. 563155 antibodies and FITC Lineage Cocktail 1 LIN 1 - CD3, CD14, CD16, CD19, CD20, CD56 Cat. No. 340546 - a lineage cocktail comprised of FITC-conjugated antibodies for the specified CD markers. Two-color flow cytometric dot plots showing the correlated expression CD141 versus Clec9A CD370 or Ig Isotype control staining were derived from Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.