BD HorizonTM BV421 Rat Anti-Human CX3CR1

Description

Multicolor flow cytometric analysis of CX3CR1 expression on human peripheral blood cells. Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with BD Horizon BUV737 Mouse Anti-Human CD56 antibody Cat. No. 564447; Top Plots , Alexa Fluor 488 Mouse Anti-Human CD3 antibody Cat. No. 557694; Bottom Plots , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plots or BD Horizon BV421 Rat Anti-Human CX3CR1 antibody Cat. No. 565800/565801; Right Plots . Two-color flow cytometric contour plots showing the correlated expression of CD56 or CD3 versus CX3CR1 or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multicolor flow cytometric analysis of CX3CR1 expression on human peripheral blood cells. Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with BD Horizon BUV737 Mouse Anti-Human CD56 antibody Cat. No. 564447; Top Plots , Alexa Fluor 488 Mouse Anti-Human CD3 antibody Cat. No. 557694; Bottom Plots , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plots or BD Horizon BV421 Rat Anti-Human CX3CR1 antibody Cat. No. 565800/565801; Right Plots . Two-color flow cytometric contour plots showing the correlated expression of CD56 or CD3 versus CX3CR1 or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Multicolor flow cytometric analysis of CX3CR1 expression on human peripheral blood cells. Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with BD Horizon BUV737 Mouse Anti-Human CD56 antibody Cat. No. 564447; Top Plots , Alexa Fluor 488 Mouse Anti-Human CD3 antibody Cat. No. 557694; Bottom Plots , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plots or BD Horizon BV421 Rat Anti-Human CX3CR1 antibody Cat. No. 565800/565801; Right Plots . Two-color flow cytometric contour plots showing the correlated expression of CD56 or CD3 versus CX3CR1 or Ig Isotype control staining , were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System.