BD HorizonTM BV421 Rat Anti-Human HLA-B

Description

Multiparameter flow cytometric analysis of HLA-B expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Human HLA-B antibody Cat. No. 569644/569645; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-B or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of HLA-B expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Human HLA-B antibody Cat. No. 569644/569645; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-B or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of HLA-B expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Human HLA-B antibody Cat. No. 569644/569645; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-B or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of HLA-B expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Human HLA-B antibody Cat. No. 569644/569645; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-B or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of HLA-B expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Human HLA-B antibody Cat. No. 569644/569645; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-B or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of HLA-B expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Human HLA-B antibody Cat. No. 569644/569645; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-B or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.