BD HorizonTM BV421 Rat Anti-Mouse Blimp-1

Description

Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553088/553087/561877 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse Blimp-1 antibody Cat. No. 564270; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553088/553087/561877 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse Blimp-1 antibody Cat. No. 564270; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553088/553087/561877 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse Blimp-1 antibody Cat. No. 564270; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553088/553087/561877 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse Blimp-1 antibody Cat. No. 564270; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553088/553087/561877 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse Blimp-1 antibody Cat. No. 564270; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with FITC Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553088/553087/561877 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse Blimp-1 antibody Cat. No. 564270; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.