BD HorizonTM BV421 Rat Anti-Mouse CD103

Description

Multicolor flow cytometric analysis of CD103 expression on mouse lymph node cells left and center panels . Lymph node cells from a C57BL/6 mouse were stained with Alexa Fluor 488 Hamster Anti-Mouse CD3e antibody Cat. No. 557666 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771; Center Panel . Two-color dot plots showing the coexpressed levels of CD103 or Ig Isotype Control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD103 expression by cells within C57BL/6 mouse intestine right panel . A mouse intestine cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored red . Images were captured on a standard four laser confocal microscope. Original magnification, 40x Multicolor flow cytometric analysis of CD103 expression on mouse lymph node cells left and center panels . Lymph node cells from a C57BL/6 mouse were stained with Alexa Fluor 488 Hamster Anti-Mouse CD3e antibody Cat. No. 557666 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771; Center Panel . Two-color dot plots showing the coexpressed levels of CD103 or Ig Isotype Control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD103 expression by cells within C57BL/6 mouse intestine right panel . A mouse intestine cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored red . Images were captured on a standard four laser confocal microscope. Original magnification, 40x Multicolor flow cytometric analysis of CD103 expression on mouse lymph node cells left and center panels . Lymph node cells from a C57BL/6 mouse were stained with Alexa Fluor 488 Hamster Anti-Mouse CD3e antibody Cat. No. 557666 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771; Center Panel . Two-color dot plots showing the coexpressed levels of CD103 or Ig Isotype Control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD103 expression by cells within C57BL/6 mouse intestine right panel . A mouse intestine cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored red . Images were captured on a standard four laser confocal microscope. Original magnification, 40x Show More Multicolor flow cytometric analysis of CD103 expression on mouse lymph node cells left and center panels . Lymph node cells from a C57BL/6 mouse were stained with Alexa Fluor 488 Hamster Anti-Mouse CD3e antibody Cat. No. 557666 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771; Center Panel . Two-color dot plots showing the coexpressed levels of CD103 or Ig Isotype Control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD103 expression by cells within C57BL/6 mouse intestine right panel . A mouse intestine cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored red . Images were captured on a standard four laser confocal microscope. Original magnification, 40x Show More Multicolor flow cytometric analysis of CD103 expression on mouse lymph node cells left and center panels . Lymph node cells from a C57BL/6 mouse were stained with Alexa Fluor 488 Hamster Anti-Mouse CD3e antibody Cat. No. 557666 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771; Center Panel . Two-color dot plots showing the coexpressed levels of CD103 or Ig Isotype Control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD103 expression by cells within C57BL/6 mouse intestine right panel . A mouse intestine cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored red . Images were captured on a standard four laser confocal microscope. Original magnification, 40x Multicolor flow cytometric analysis of CD103 expression on mouse lymph node cells left and center panels . Lymph node cells from a C57BL/6 mouse were stained with Alexa Fluor 488 Hamster Anti-Mouse CD3e antibody Cat. No. 557666 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Panel or with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771; Center Panel . Two-color dot plots showing the coexpressed levels of CD103 or Ig Isotype Control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD103 expression by cells within C57BL/6 mouse intestine right panel . A mouse intestine cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD103 antibody Cat. No. 562771, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored red . Images were captured on a standard four laser confocal microscope. Original magnification, 40x