
564347 ·
BDB Bioscience · Cat: 564347
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were washed and subsequently stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse CD107a antibody Cat. No. 564347; solid line histogram . Fluorescence histograms showing the expression of CD107a or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were washed and subsequently stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse CD107a antibody Cat. No. 564347; solid line histogram . Fluorescence histograms showing the expression of CD107a or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD107a expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were washed and subsequently stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse CD107a antibody Cat. No. 564347; solid line histogram . Fluorescence histograms showing the expression of CD107a or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.