BD HorizonTM BV421 Rat anti-Mouse CD150

Description

Multicolor flow cytometric analysis of CD150 expression by adult mouse spleen cells and bone marrow hematopoietic stem cells. Panel A BALB/c spleen cells were stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/553089/561878 and Brilliant Violet 421 Rat anti-Mouse CD150 Cat. No. 562811 , staining cells well above background compared to Brilliant Violet 421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 data not shown . A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by gated events with the forward and side-light scattering characteristics of viable lymphocytes. Panel B BALB/c mouse bone-marrow cells were labeled with the BD IMag Mouse Hematopoietic Progenitor Enrichment Set Cat. No. 558451 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the set protocol. The non-depleted bone marrow cells were subsequently stained with APC Mouse Lineage Antibody Cocktail Cat. No. 558074 , FITC Rat Anti-Mouse CD41 Cat. No. 553848/561849 , APC-Cy7 Hamster Anti-Mouse CD48 Cat. No. 561242 and Brilliant Violet 421 Rat anti-Mouse CD150 antibodies. A fluorescence histogram Right Plot shows little or no CD41 expression P4 Gate by bone marrow cells that were progressively gated as viable light-scatter gated . Lineage-negative CD48- CD150+ Middle Plot, P3 Gate bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD150 expression by adult mouse spleen cells and bone marrow hematopoietic stem cells. Panel A BALB/c spleen cells were stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/553089/561878 and Brilliant Violet 421 Rat anti-Mouse CD150 Cat. No. 562811 , staining cells well above background compared to Brilliant Violet 421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 data not shown . A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by gated events with the forward and side-light scattering characteristics of viable lymphocytes. Panel B BALB/c mouse bone-marrow cells were labeled with the BD IMag Mouse Hematopoietic Progenitor Enrichment Set Cat. No. 558451 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the set protocol. The non-depleted bone marrow cells were subsequently stained with APC Mouse Lineage Antibody Cocktail Cat. No. 558074 , FITC Rat Anti-Mouse CD41 Cat. No. 553848/561849 , APC-Cy7 Hamster Anti-Mouse CD48 Cat. No. 561242 and Brilliant Violet 421 Rat anti-Mouse CD150 antibodies. A fluorescence histogram Right Plot shows little or no CD41 expression P4 Gate by bone marrow cells that were progressively gated as viable light-scatter gated . Lineage-negative CD48- CD150+ Middle Plot, P3 Gate bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD150 expression by adult mouse spleen cells and bone marrow hematopoietic stem cells. Panel A BALB/c spleen cells were stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/553089/561878 and Brilliant Violet 421 Rat anti-Mouse CD150 Cat. No. 562811 , staining cells well above background compared to Brilliant Violet 421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 data not shown . A two-color flow cytometric dot plot shows the expression of B220 versus CD150 expressed by gated events with the forward and side-light scattering characteristics of viable lymphocytes. Panel B BALB/c mouse bone-marrow cells were labeled with the BD IMag Mouse Hematopoietic Progenitor Enrichment Set Cat. No. 558451 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the set protocol. The non-depleted bone marrow cells were subsequently stained with APC Mouse Lineage Antibody Cocktail Cat. No. 558074 , FITC Rat Anti-Mouse CD41 Cat. No. 553848/561849 , APC-Cy7 Hamster Anti-Mouse CD48 Cat. No. 561242 and Brilliant Violet 421 Rat anti-Mouse CD150 antibodies. A fluorescence histogram Right Plot shows little or no CD41 expression P4 Gate by bone marrow cells that were progressively gated as viable light-scatter gated . Lineage-negative CD48- CD150+ Middle Plot, P3 Gate bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System.