BD HorizonTM BV421 Rat Anti-Mouse CD169

Description

Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 553129/561083 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD169 antibody Cat. No. 566604/566606; Right Plot at 1 mug/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G Gr-1 versus CD169 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 553129/561083 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD169 antibody Cat. No. 566604/566606; Right Plot at 1 mug/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G Gr-1 versus CD169 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 553129/561083 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD169 antibody Cat. No. 566604/566606; Right Plot at 1 mug/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G Gr-1 versus CD169 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 553129/561083 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD169 antibody Cat. No. 566604/566606; Right Plot at 1 mug/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G Gr-1 versus CD169 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 553129/561083 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD169 antibody Cat. No. 566604/566606; Right Plot at 1 mug/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G Gr-1 versus CD169 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 553129/561083 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD169 antibody Cat. No. 566604/566606; Right Plot at 1 mug/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G Gr-1 versus CD169 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.