BD HorizonTM BV421 Rat Anti-Mouse CD200R3

Description

Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD200R3 antibody Cat. No. 567833; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD200R3 antibody Cat. No. 567833; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD200R3 antibody Cat. No. 567833; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD200R3 antibody Cat. No. 567833; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD200R3 antibody Cat. No. 567833; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD200R3 antibody Cat. No. 567833; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.