BD HorizonTM BV421 Rat Anti-Mouse CD209b

Description

Multicolor flow cytometric analysis of CD209b expression on BALB/c versus C57BL/6 Mouse peritoneal exudate cells PECs . Resident peritoneal cells from BALB/c Top Plots or C57BL/6 Bottom Plots mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-CD11b antibody Cat. No. 557397/561689 and with either BD Horizon BV421 Rat IgM, kappa Isotype Control Cat. No. 562708; Left Plots or BD Horizon BV421 Rat Anti-Mouse CD209b antibody Cat. No. 569015/569016; Right Plots at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD209b or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD209b expression on BALB/c versus C57BL/6 Mouse peritoneal exudate cells PECs . Resident peritoneal cells from BALB/c Top Plots or C57BL/6 Bottom Plots mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-CD11b antibody Cat. No. 557397/561689 and with either BD Horizon BV421 Rat IgM, kappa Isotype Control Cat. No. 562708; Left Plots or BD Horizon BV421 Rat Anti-Mouse CD209b antibody Cat. No. 569015/569016; Right Plots at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD209b or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD209b expression on BALB/c versus C57BL/6 Mouse peritoneal exudate cells PECs . Resident peritoneal cells from BALB/c Top Plots or C57BL/6 Bottom Plots mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-CD11b antibody Cat. No. 557397/561689 and with either BD Horizon BV421 Rat IgM, kappa Isotype Control Cat. No. 562708; Left Plots or BD Horizon BV421 Rat Anti-Mouse CD209b antibody Cat. No. 569015/569016; Right Plots at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD209b or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.