BD HorizonTM BV421 Rat Anti-Mouse CD21/CD35

Description

Flow cytometric analysis of CD21/CD35 expression on mouse splenic leucocytes. Mouse splenocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Rat Anti-Mouse CD45R/B220 antibody Cat No. 567381 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat No. 562602, Left Plot or BD Horizon BV421 Rat Anti-Mouse CD21/CD35 antibody Cat No. 568301/568302; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD21/CD35 expression on mouse splenic leucocytes. Mouse splenocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Rat Anti-Mouse CD45R/B220 antibody Cat No. 567381 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat No. 562602, Left Plot or BD Horizon BV421 Rat Anti-Mouse CD21/CD35 antibody Cat No. 568301/568302; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD21/CD35 expression on mouse splenic leucocytes. Mouse splenocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Rat Anti-Mouse CD45R/B220 antibody Cat No. 567381 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat No. 562602, Left Plot or BD Horizon BV421 Rat Anti-Mouse CD21/CD35 antibody Cat No. 568301/568302; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD21/CD35 expression on mouse splenic leucocytes. Mouse splenocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Rat Anti-Mouse CD45R/B220 antibody Cat No. 567381 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat No. 562602, Left Plot or BD Horizon BV421 Rat Anti-Mouse CD21/CD35 antibody Cat No. 568301/568302; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD21/CD35 expression on mouse splenic leucocytes. Mouse splenocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Rat Anti-Mouse CD45R/B220 antibody Cat No. 567381 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat No. 562602, Left Plot or BD Horizon BV421 Rat Anti-Mouse CD21/CD35 antibody Cat No. 568301/568302; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD21/CD35 expression on mouse splenic leucocytes. Mouse splenocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon R718 Rat Anti-Mouse CD45R/B220 antibody Cat No. 567381 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat No. 562602, Left Plot or BD Horizon BV421 Rat Anti-Mouse CD21/CD35 antibody Cat No. 568301/568302; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD21/CD35 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.