BD HorizonTM BV421 Rat Anti-Mouse CD273 (PD-L2)

Description

Multicolor flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 700 Hamster Anti-Mouse CD11c antibody Cat. No. 560583 and either BD HorizonBV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD HorizonBV421 Rat Anti-Mouse CD273 antibody Cat. No. 567374; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 700 Hamster Anti-Mouse CD11c antibody Cat. No. 560583 and either BD HorizonBV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD HorizonBV421 Rat Anti-Mouse CD273 antibody Cat. No. 567374; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 700 Hamster Anti-Mouse CD11c antibody Cat. No. 560583 and either BD HorizonBV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD HorizonBV421 Rat Anti-Mouse CD273 antibody Cat. No. 567374; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 700 Hamster Anti-Mouse CD11c antibody Cat. No. 560583 and either BD HorizonBV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD HorizonBV421 Rat Anti-Mouse CD273 antibody Cat. No. 567374; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 700 Hamster Anti-Mouse CD11c antibody Cat. No. 560583 and either BD HorizonBV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD HorizonBV421 Rat Anti-Mouse CD273 antibody Cat. No. 567374; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD273 PD-L2 expression on mouse splenic dendritic cells. BALB/c mouse splenic leucocytes were cultured overnight with Recombinant Mouse GM-CSF protein Cat. No. 554586; 5 ng/ml . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 700 Hamster Anti-Mouse CD11c antibody Cat. No. 560583 and either BD HorizonBV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD HorizonBV421 Rat Anti-Mouse CD273 antibody Cat. No. 567374; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD273 PD-L2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.