BD HorizonTM BV421 Rat Anti-Mouse CD274

Description

Two-color flow cytometric analysis of CD274 expression on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated Upper Panels or activated Lower Panels by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553729/557307/561828 and either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Panels or BD Horizon BV421 Rat Anti-Mouse CD274 antibody Cat. No. 564716; Right Panels . Two-color flow cytometric contour plots showing the correlated expression of CD274 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD274 expression on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated Upper Panels or activated Lower Panels by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553729/557307/561828 and either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Panels or BD Horizon BV421 Rat Anti-Mouse CD274 antibody Cat. No. 564716; Right Panels . Two-color flow cytometric contour plots showing the correlated expression of CD274 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD274 expression on resting and activated mouse splenocytes. Mouse splenic leucocytes were either not activated Upper Panels or activated Lower Panels by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 antibody Cat. No. 553057 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553729/557307/561828 and either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Panels or BD Horizon BV421 Rat Anti-Mouse CD274 antibody Cat. No. 564716; Right Panels . Two-color flow cytometric contour plots showing the correlated expression of CD274 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.