BD HorizonTM BV421 Rat Anti-Mouse CD274 (PD-L1)

Description

Multicolor flow cytometric analysis of CD274 PD-L1 expression on resting and activated mouse splenocytes. BALB/c mouse splenic leucocytes were either not activated Upper Plots or activated Lower Plots by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553049 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plots or BD Horizon BV421 Rat Anti-Mouse CD274 PD-L1 antibody Cat. No. 568309/568310; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD274 PD-L1 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD274 PD-L1 expression on resting and activated mouse splenocytes. BALB/c mouse splenic leucocytes were either not activated Upper Plots or activated Lower Plots by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553049 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plots or BD Horizon BV421 Rat Anti-Mouse CD274 PD-L1 antibody Cat. No. 568309/568310; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD274 PD-L1 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD274 PD-L1 expression on resting and activated mouse splenocytes. BALB/c mouse splenic leucocytes were either not activated Upper Plots or activated Lower Plots by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 553049 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plots or BD Horizon BV421 Rat Anti-Mouse CD274 PD-L1 antibody Cat. No. 568309/568310; Right Plots at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD274 PD-L1 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light- scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.