BD HorizonTM BV421 Rat Anti-Mouse CD3 Molecular Complex

Description

Immunohistofluorescent analysis of CD3 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564008, pseudo-colored green and Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557683, pseudo-colored red . Images were captured on a standard epifluorescence. Original magnification, 20x Two-color flow cytometric analysis of CD3 Molecular Complex expression on mouse splenocytes. Splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 557399/553786/561736 and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564008; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of CD3 or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunohistofluorescent analysis of CD3 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564008, pseudo-colored green and Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557683, pseudo-colored red . Images were captured on a standard epifluorescence. Original magnification, 20x Show More Two-color flow cytometric analysis of CD3 Molecular Complex expression on mouse splenocytes. Splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 557399/553786/561736 and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564008; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of CD3 or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Immunohistofluorescent analysis of CD3 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564008, pseudo-colored green and Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557683, pseudo-colored red . Images were captured on a standard epifluorescence. Original magnification, 20x Two-color flow cytometric analysis of CD3 Molecular Complex expression on mouse splenocytes. Splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 557399/553786/561736 and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564008; Right Panel . Two-color flow cytometric dot plots show the correlated expression patterns of CD3 or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.