BD HorizonTM BV421 Rat Anti-Mouse CD301b (Mgl2)

Description

Multicolor flow cytometric analysis of CD301b Mgl2 expression on Mouse bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells DCs from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD11c antibody Cat. No. 557401 and with either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD301b Mgl2 antibody Cat. No. 569535/569536; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b Mgl2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD301b Mgl2 expression on Mouse bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells DCs from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD11c antibody Cat. No. 557401 and with either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD301b Mgl2 antibody Cat. No. 569535/569536; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b Mgl2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD301b Mgl2 expression on Mouse bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells DCs from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD11c antibody Cat. No. 557401 and with either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD301b Mgl2 antibody Cat. No. 569535/569536; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b Mgl2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD301b Mgl2 expression on Mouse bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells DCs from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD11c antibody Cat. No. 557401 and with either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD301b Mgl2 antibody Cat. No. 569535/569536; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b Mgl2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD301b Mgl2 expression on Mouse bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells DCs from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD11c antibody Cat. No. 557401 and with either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD301b Mgl2 antibody Cat. No. 569535/569536; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b Mgl2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD301b Mgl2 expression on Mouse bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells DCs from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Hamster Anti-Mouse CD11c antibody Cat. No. 557401 and with either BD Horizon BV421 Rat IgG2a, lambda Isotype Control Cat. No. 562965; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD301b Mgl2 antibody Cat. No. 569535/569536; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b Mgl2 or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.