BD HorizonTM BV421 Rat Anti-Mouse CD326

Description

Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 or BD Horizon BV421 Rat Anti-Mouse CD326 antibody Cat. No. 563214 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line and Ig Isotype Control dashed line fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No 553062/553061/561827 and PE Rat Anti-Mouse CD25 Cat. No. 553866/561065 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 or BD Horizon BV421 Rat Anti-Mouse CD326 antibody Cat. No. 563214 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line and Ig Isotype Control dashed line fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No 553062/553061/561827 and PE Rat Anti-Mouse CD25 Cat. No. 553866/561065 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 or BD Horizon BV421 Rat Anti-Mouse CD326 antibody Cat. No. 563214 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line and Ig Isotype Control dashed line fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No 553062/553061/561827 and PE Rat Anti-Mouse CD25 Cat. No. 553866/561065 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 or BD Horizon BV421 Rat Anti-Mouse CD326 antibody Cat. No. 563214 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line and Ig Isotype Control dashed line fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No 553062/553061/561827 and PE Rat Anti-Mouse CD25 Cat. No. 553866/561065 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 or BD Horizon BV421 Rat Anti-Mouse CD326 antibody Cat. No. 563214 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line and Ig Isotype Control dashed line fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No 553062/553061/561827 and PE Rat Anti-Mouse CD25 Cat. No. 553866/561065 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 or BD Horizon BV421 Rat Anti-Mouse CD326 antibody Cat. No. 563214 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line and Ig Isotype Control dashed line fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No 553062/553061/561827 and PE Rat Anti-Mouse CD25 Cat. No. 553866/561065 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.