BD HorizonTM BV421 Rat Anti-Mouse CD366 (TIM-3)

Description

Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553032 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 569808/569882; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553032 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 569808/569882; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553032 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 569808/569882; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553032 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 569808/569882; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553032 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 569808/569882; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553032 and with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 569808/569882; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.