BD HorizonTM BV421 Rat Anti-Mouse CD368 (Clec4d)

Description

Multicolor flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571503/571553; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571503/571553; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571503/571553; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571503/571553; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571503/571553; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen PE Rat Anti-Mouse Ly-6G antibody Cat. No. 551461 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD Horizon BV421 Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571503/571553; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.