BD HorizonTM BV421 Rat Anti-Mouse CD370 (Clec9A)

Description

Multicolor flow cytometric analysis of CD370 Clec9A expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 , FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 , APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies and either BD Horizon BV421 Rat IgG2a kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD370 Clec9A antibody Cat. No. 564271; Right Panel . Two color flow cytometric contour plots showing the correlated expression of CD11c and Clec9A or Ig Isotype control staining were derived from CD4- CD8a+ gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD370 Clec9A expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 , FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 , APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies and either BD Horizon BV421 Rat IgG2a kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD370 Clec9A antibody Cat. No. 564271; Right Panel . Two color flow cytometric contour plots showing the correlated expression of CD11c and Clec9A or Ig Isotype control staining were derived from CD4- CD8a+ gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD370 Clec9A expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 , FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 , APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies and either BD Horizon BV421 Rat IgG2a kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD370 Clec9A antibody Cat. No. 564271; Right Panel . Two color flow cytometric contour plots showing the correlated expression of CD11c and Clec9A or Ig Isotype control staining were derived from CD4- CD8a+ gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD370 Clec9A expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 , FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 , APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies and either BD Horizon BV421 Rat IgG2a kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD370 Clec9A antibody Cat. No. 564271; Right Panel . Two color flow cytometric contour plots showing the correlated expression of CD11c and Clec9A or Ig Isotype control staining were derived from CD4- CD8a+ gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD370 Clec9A expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 , FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 , APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies and either BD Horizon BV421 Rat IgG2a kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD370 Clec9A antibody Cat. No. 564271; Right Panel . Two color flow cytometric contour plots showing the correlated expression of CD11c and Clec9A or Ig Isotype control staining were derived from CD4- CD8a+ gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD370 Clec9A expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 , FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 , APC Hamster Anti-Mouse CD11c Cat. No. 550261/561119 antibodies and either BD Horizon BV421 Rat IgG2a kappa Isotype Control Cat. No. 562602; Left Panel or BD Horizon BV421 Rat Anti-Mouse CD370 Clec9A antibody Cat. No. 564271; Right Panel . Two color flow cytometric contour plots showing the correlated expression of CD11c and Clec9A or Ig Isotype control staining were derived from CD4- CD8a+ gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.