BD HorizonTM BV421 Rat anti-Mouse CD371 (Clec12A)

Description

Multicolor flow cytometric analysis of CD371 Clec12A expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , PerCP-Cy5.5 Hamster Anti-Mouse CD11c Cat. No. 560584 , APC-H7 Rat Anti-Mouse CD19 Cat. No. 560143/560245 , PE Rat Anti-Mouse CD45R/B220 Cat. No. 553089/553090/561878 , and BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 antibodies and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD371 Clec12A antibody Cat. No. 564795; solid line histograms . Overlapping histograms showing CD371 expression or Ig Isotype control staining on either CD11c-high CD45R/B220- Left Panel or CD11c-intermediate CD45R/B220+ Right Panel cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD371 Clec12A expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , PerCP-Cy5.5 Hamster Anti-Mouse CD11c Cat. No. 560584 , APC-H7 Rat Anti-Mouse CD19 Cat. No. 560143/560245 , PE Rat Anti-Mouse CD45R/B220 Cat. No. 553089/553090/561878 , and BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 antibodies and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD371 Clec12A antibody Cat. No. 564795; solid line histograms . Overlapping histograms showing CD371 expression or Ig Isotype control staining on either CD11c-high CD45R/B220- Left Panel or CD11c-intermediate CD45R/B220+ Right Panel cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD371 Clec12A expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , PerCP-Cy5.5 Hamster Anti-Mouse CD11c Cat. No. 560584 , APC-H7 Rat Anti-Mouse CD19 Cat. No. 560143/560245 , PE Rat Anti-Mouse CD45R/B220 Cat. No. 553089/553090/561878 , and BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 antibodies and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD371 Clec12A antibody Cat. No. 564795; solid line histograms . Overlapping histograms showing CD371 expression or Ig Isotype control staining on either CD11c-high CD45R/B220- Left Panel or CD11c-intermediate CD45R/B220+ Right Panel cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD371 Clec12A expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , PerCP-Cy5.5 Hamster Anti-Mouse CD11c Cat. No. 560584 , APC-H7 Rat Anti-Mouse CD19 Cat. No. 560143/560245 , PE Rat Anti-Mouse CD45R/B220 Cat. No. 553089/553090/561878 , and BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 antibodies and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD371 Clec12A antibody Cat. No. 564795; solid line histograms . Overlapping histograms showing CD371 expression or Ig Isotype control staining on either CD11c-high CD45R/B220- Left Panel or CD11c-intermediate CD45R/B220+ Right Panel cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD371 Clec12A expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , PerCP-Cy5.5 Hamster Anti-Mouse CD11c Cat. No. 560584 , APC-H7 Rat Anti-Mouse CD19 Cat. No. 560143/560245 , PE Rat Anti-Mouse CD45R/B220 Cat. No. 553089/553090/561878 , and BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 antibodies and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD371 Clec12A antibody Cat. No. 564795; solid line histograms . Overlapping histograms showing CD371 expression or Ig Isotype control staining on either CD11c-high CD45R/B220- Left Panel or CD11c-intermediate CD45R/B220+ Right Panel cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD371 Clec12A expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , PerCP-Cy5.5 Hamster Anti-Mouse CD11c Cat. No. 560584 , APC-H7 Rat Anti-Mouse CD19 Cat. No. 560143/560245 , PE Rat Anti-Mouse CD45R/B220 Cat. No. 553089/553090/561878 , and BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 antibodies and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD371 Clec12A antibody Cat. No. 564795; solid line histograms . Overlapping histograms showing CD371 expression or Ig Isotype control staining on either CD11c-high CD45R/B220- Left Panel or CD11c-intermediate CD45R/B220+ Right Panel cells were derived from gated events with the forward and side light-scatter characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.