BD HorizonTM BV421 Rat Anti-Mouse CD39

Description

Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c Mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553729, 557307, or 561828 , APC Rat Anti-Mouse CD25 Cat. No. 557192 or 561048 , and BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603 or BD Horizon BV421 Rat Anti-Mouse CD39 antibody Cat. No. 567105 at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Upper panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 right or Ig Isotype control left versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 right or Ig Isotype control left versus CD25 staining were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c Mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553729, 557307, or 561828 , APC Rat Anti-Mouse CD25 Cat. No. 557192 or 561048 , and BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603 or BD Horizon BV421 Rat Anti-Mouse CD39 antibody Cat. No. 567105 at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Upper panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 right or Ig Isotype control left versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 right or Ig Isotype control left versus CD25 staining were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c Mouse splenocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553729, 557307, or 561828 , APC Rat Anti-Mouse CD25 Cat. No. 557192 or 561048 , and BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603 or BD Horizon BV421 Rat Anti-Mouse CD39 antibody Cat. No. 567105 at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Upper panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 right or Ig Isotype control left versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower panels: Two-parameter flow cytometric pseudocolor plots showing correlated expression of CD39 right or Ig Isotype control left versus CD25 staining were derived from CD4-positive viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.