BD HorizonTM BV421 Rat Anti-Mouse CD68

Description

Flow cytometric analysis of CD68 expression by activated F4/80-positive mouse peritoneal macrophages. C57BL/6 mouse thioglycolate-elicited peritoneal exudate cells PECs were harvested and fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse CD68 antibody Cat. No. 566388/566389; solid line histogram at 0.25 mug/test. The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from F4/80 positive-gated events with the forward and side light-scatter characteristics of intact PECs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD68 expression by activated F4/80-positive mouse peritoneal macrophages. C57BL/6 mouse thioglycolate-elicited peritoneal exudate cells PECs were harvested and fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse CD68 antibody Cat. No. 566388/566389; solid line histogram at 0.25 mug/test. The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from F4/80 positive-gated events with the forward and side light-scatter characteristics of intact PECs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD68 expression by activated F4/80-positive mouse peritoneal macrophages. C57BL/6 mouse thioglycolate-elicited peritoneal exudate cells PECs were harvested and fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD Horizon BV421 Rat Anti-Mouse CD68 antibody Cat. No. 566388/566389; solid line histogram at 0.25 mug/test. The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from F4/80 positive-gated events with the forward and side light-scatter characteristics of intact PECs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.