BD HorizonTM BV421 Rat Anti-Mouse CD86

Description

Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD86 antibody Cat. No. 564198; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD86 antibody Cat. No. 564198; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD86 antibody Cat. No. 564198; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD86 antibody Cat. No. 564198; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD86 antibody Cat. No. 564198; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD86 antibody Cat. No. 564198; solid line histograms . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.