BD HorizonTM BV421 Rat Anti-Mouse F4/80

Description

Two-color flow cytometric analysis of mouse F4/80 expression. Panel 1 - Spleen. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with Alexa Fluor 647 Rat Anti-Mouse CD11b antibody Cat. No. 557686 and either BD Horizon BV421 Rat IgG2a Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse F4/80 antibody Cat. No. 565411; Right Plot . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Panel 2 - Peritoneal. C57BL/6 mouse peritoneal cells were preincubated with Mouse BD Fc Block and stained with APC Rat Anti-Mouse CD117 Cat. No. 553356/561074 and BV421 Rat Anti-Mouse F4/80 antibodies. The two-color contour plot showing the correlated expression of F4/80 versus CD117 was derived from gated events with the light-scatter characteristics of viable peritoneal cells. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Image analysis of F4/80 expression in mouse spleen. Panel 3 - Spleen. A section from a paraformaldehyde-fixed and frozen normal C57BL/6 mouse spleen was stained with BV421 Rat Anti-Mouse F4/80 pseudo-colored green , Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 Cat. No. 557683; pseudo-colored red , and BD Horizon BV480 Anti-CD3 pseudo-colored aqua antibodies. The image was captured on a standard Epifluorescence microscope using 392/23nm excitation and 430/24nm emission. Two-color flow cytometric analysis of mouse F4/80 expression. Panel 1 - Spleen. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with Alexa Fluor 647 Rat Anti-Mouse CD11b antibody Cat. No. 557686 and either BD Horizon BV421 Rat IgG2a Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse F4/80 antibody Cat. No. 565411; Right Plot . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Panel 2 - Peritoneal. C57BL/6 mouse peritoneal cells were preincubated with Mouse BD Fc Block and stained with APC Rat Anti-Mouse CD117 Cat. No. 553356/561074 and BV421 Rat Anti-Mouse F4/80 antibodies. The two-color contour plot showing the correlated expression of F4/80 versus CD117 was derived from gated events with the light-scatter characteristics of viable peritoneal cells. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Image analysis of F4/80 expression in mouse spleen. Panel 3 - Spleen. A section from a paraformaldehyde-fixed and frozen normal C57BL/6 mouse spleen was stained with BV421 Rat Anti-Mouse F4/80 pseudo-colored green , Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 Cat. No. 557683; pseudo-colored red , and BD Horizon BV480 Anti-CD3 pseudo-colored aqua antibodies. The image was captured on a standard Epifluorescence microscope using 392/23nm excitation and 430/24nm emission. Show More Two-color flow cytometric analysis of mouse F4/80 expression. Panel 1 - Spleen. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with Alexa Fluor 647 Rat Anti-Mouse CD11b antibody Cat. No. 557686 and either BD Horizon BV421 Rat IgG2a Isotype Control Cat. No. 562602; Left Plot or BD Horizon BV421 Rat Anti-Mouse F4/80 antibody Cat. No. 565411; Right Plot . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Panel 2 - Peritoneal. C57BL/6 mouse peritoneal cells were preincubated with Mouse BD Fc Block and stained with APC Rat Anti-Mouse CD117 Cat. No. 553356/561074 and BV421 Rat Anti-Mouse F4/80 antibodies. The two-color contour plot showing the correlated expression of F4/80 versus CD117 was derived from gated events with the light-scatter characteristics of viable peritoneal cells. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Image analysis of F4/80 expression in mouse spleen. Panel 3 - Spleen. A section from a paraformaldehyde-fixed and frozen normal C57BL/6 mouse spleen was stained with BV421 Rat Anti-Mouse F4/80 pseudo-colored green , Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 Cat. No. 557683; pseudo-colored red , and BD Horizon BV480 Anti-CD3 pseudo-colored aqua antibodies. The image was captured on a standard Epifluorescence microscope using 392/23nm excitation and 430/24nm emission.