BD HorizonTM BV421 Rat Anti-Mouse Foxp3
562996 ·
BDB Bioscience · Cat: 562996
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. Splenic leucocytes from BALB/c mice were stained with APC Rat anti-Mouse CD4 antibody Cat. No. 553051/561091 , and fixed and permeabilized using working solutions of the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421Rat Anti-Mouse Foxp3 antibody Cat. No. 562996 . The flow cytometric dot plot shows the correlated expression of Foxp3 versus CD4 for gated events with the forward and side light-scattering characteristics of intact leucocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. Splenic leucocytes from BALB/c mice were stained with APC Rat anti-Mouse CD4 antibody Cat. No. 553051/561091 , and fixed and permeabilized using working solutions of the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421Rat Anti-Mouse Foxp3 antibody Cat. No. 562996 . The flow cytometric dot plot shows the correlated expression of Foxp3 versus CD4 for gated events with the forward and side light-scattering characteristics of intact leucocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. Splenic leucocytes from BALB/c mice were stained with APC Rat anti-Mouse CD4 antibody Cat. No. 553051/561091 , and fixed and permeabilized using working solutions of the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421Rat Anti-Mouse Foxp3 antibody Cat. No. 562996 . The flow cytometric dot plot shows the correlated expression of Foxp3 versus CD4 for gated events with the forward and side light-scattering characteristics of intact leucocytes. Flow cytometry was performed using a BD LSRFortessa Cell Analyzer System.